HEMEROCALLIS CITRINA BARONI AS A PROMISING SOURCE OF BIOLOGICALLY ACTIVE SUBSTANCES WITH ANTIMICROBIAL EFFECT
DOI:
https://doi.org/10.32782/2786-9067-2025-29-5Keywords:
Hemerocallis citrina Baroni, aqueous-ethanolic extracts of leaves and flowers, antimicrobial activityAbstract
Various classes of plant phenolic compounds are considered to be beneficial for health and indispensable components in nutraceuticals, pharmaceuticals, medicine, cosmetics and other applications, in particular as those with antimicrobial properties. Therefore, it is important to search for alternative agents with antimicrobial activity, among which introduced plants arepotential. In particular, the species Hemerocallis citrina Baroni has been introduced in Ukraine, and it is advisable to conduct a comprehen- sive study of it.The aim of the work was to investigate the phenolics’, flavonoids’, and hydroxycinnamic acids’ content, and antimicrobial activity of the aquatic and hydroalcoholic extracts of the flowers and leaves of Hemero- callis citrina Baroni.Materials and methods. Plant parts were extracted with20%, 60% 96% aqueous ethanol (AE) in reflux conditions to obtain the crude extracts. Phenolic content was determined by the Folin-Ciocalteu meth- od, flavonoids and hydroxycinnamic acids spectrophotomically. The antimicrobial activity of extracts was evaluated against Bacillus subtilis, B. brevis, Candida parapsilosis and C. pseudotropicalis. The zone of inhibition of the plant extracts were evaluated by the agar diffusion disc method.Research results. Total phenolic and flavonoid content of the extracts was higher in leaves (5,772–11,076 mg·g -1 DW in GAE) than in flowers (4,096–6,156 mg·g -1 DW in GAE). It was found that H. citrina leaf extracts prepared with 60- and 95% AE caused ZI of 25,7 ± 0,5 and 20,0 ± 0,8 mm on the path- ogenic bacteria B. cereus, respectively. The diameter of the inhibition zones of leaf extracts prepared with 20, 60 and 96% AE against B. subtilis was 11,7–16,3 mm. Against B. brevis, high activity was observed in extracts with 60 and 96% AE: 24,7 and 18,3 mm, respectively. Only extracts with 96% AE had a significant effect on Candida parapsilosis and C. pseudotropicalis: 24,0 and 21,3 mm ZI, respectively. According to the results of correlation analysis, statistically significant relationships (р < 0,01) were found between the zones of inhibition of C. parapsilosis and flavonoids of H. citrina leaf extract with 60% AE, as well as between C. pseudotropicalis ZI and hydroxycinnamic acids (in terms of chlorogenic acid) of H. citrina leaf extract with 20% AE.Conclusion. The present research may contribute to demonstrating that H. citrina is a source of com- pounds for the development of new therapeutic drugs with antimicrobial activity.
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