FEATURES OF ADHESION MICROORGANISMS TO EXPERIMENTAL SAMPLES OF BASIC POLYMERS DEPENDING ON SURFACE TREATMENT WITH DIFFERENT POLISHING PASTES
DOI:
https://doi.org/10.32782/3041-1394.2025-1.5Keywords:
thermoplastic polymers, acrylic polymers, scanning microscopy, adhesion of microorganismsAbstract
Introduction. The high need for orthopedic treatment with removable dentures, as before, is led by insufficient sanitation of the oral cavity and untimely referral of patients to the dentist. One of the main factors in the successful treatment of patients with partial tooth loss is selectivity in the choice of polymer base materials for removable dentures. It is important how resistant the selected material is to the biological environment of the oral cavity, since low resistance can lead to changes in the quantitative and qualitative composition of the microflora. In addition, it is necessary to adhere to the technology of manufacturing removable dentures, since the formation of roughness, pores in the denture, insufficient care of the denture promotes the penetration of oral microorganisms into the base of the denture, stimulating the formation of plaque on the surface of the denture, which contains carbohydrates, proteins, leukocytes. Despite the fact that numerous studies have been devoted to studying the impact of removable dentures on the biocenosis of the oral cavity, the issue of the impact of the final treatment of the denture base with polishing pastes on the ability of microbial adhesion to its surface remains incompletely understood. The aim of the study. Was to investigate the main surface parameters of acrylic and thermoplastic polymers using scanning microscopy and primary adhesion of microorganisms, taking into account the treatment of samples with different polishing pastes. Research materials and methods. The studied samples of basic polymers were treated with various polishing pastes. For the experiment of modeling the primary adhesion of microorganisms to material samples, strains of opportunistic microorganisms were used. Electron microscopic studies of materials were performed using a scanning electron microscope. Results. According to scanning microscopy data studied polymer materials, it was determined that smoother surfaces after polishing with Vertex™ThermoGloss paste obtained from thermoplastic Vertex™ ThermoSens material compared to Blue Shine paste, and acrylic ones Villacryl H Plus polymers revealed fewer surface defects than in polymer Ftorax. Thermoplastic and acrylic polymer samples for the manufacture of removable denture bases have different degrees of microbial adhesion, which depends on the choice of polishing paste. Conclusions. Polishing the surface of thermoplastic and acrylic polymers with Vertex™ThermoGloss paste significantly affects the reduction of primary adhesion of microorganisms, and a particularly pronounced decrease in adhesion is noted in Candida albicans fungi.
References
Yanishen IV, Sokhan NV, Fedotova EL, Sokhan MN. Microecology of the oral cavity in the period of adaptation to removable dentures. World Science. 2017;4(20(6)):13–7.
Joseph A, Mahajan H, Somkuwar K, Yadav NS, Saxena V, Verma V. Analysis of denture base displacement between conventional acrylic removable partial dentures and click fit partials for Kennedy’s class Ι and ΙΙ situations: an in vitro study. The Journal of Contemporary Dental Practic. 2022;23(3):351–4.
Кордіяк АЮ, Братусь-Гриньків РР. Оцінка якості життя пацієнтів після ортопедичного лікування з використанням опитувальника стоматологічного профільного. Український стоматологічний альманах. 2021;2:52–58.
Гуньовський ЯР, Макєєв ВФ. Особливості адаптації пацієнтів до часткових знімних протезів на основі динамічного вивчення стану слизової оболонки порожнини рота в нових умовах функціонування. Сучасна стоматологія. 2022;3–4:33–7.
Кузь ВС, Дворник ВМ, Кузь ГМ. Характеристика сучасних базисних стоматологічних матеріалів та їх вплив на тканини порожнини рота. Актуальні проблеми сучасної медицини: Вісник Української медичної стоматологічної академії. 2015;14(2(46)):179–184.
Макєєв ВФ, Гуньовський ЯР. Microbiological explanation of choosing the bases materials for removable dentures. Środowisko a stan zdrowia jamy ustnej; 12 maj 2022; Lublin. Lublin: Uniwersytet medyczny w Lublinie; 2022. P. 20.
Макєєв ВФ, Гуньовський ЯР, Гуньовська РП. Результати вивчення особливостей поверхневої структури стоматологічних полімерів для знімного протезування методом скануючої мікроскопії після їх обробки різними полірувальними пастами. Сучасна стоматологія. 2020;1(100):7–11.
Hariri MA, Sayed ME. Effect of nylon and acetal denture base material on Candida ablicans count for partial denture cases. Future Dental Journal. 2020;5(2):1–5.
Kraft J, Hanck H, Neidermeir W. Effect of denture adhesives on growth of Candida species. Deutsch. Zahnarzt. Z. 2014;11:885-7.
Fouda SM, Gad MM, Ellakany P, Al-Thobity AM, Al-Harbi FA, Virtanen JI. [et. al.] The effect of nanodiamonds on candida albicans adhesion and surface characteristics of PMMA denture base material – an in vitro study. Journal of Applied. Oral Science. 2019. DOI: 10.1590/1678-7757-2018-0779.
Han L, Ishizaki H, Fukushima M, Okiji T. Morphological analysis of flowable resins after long-term storage or surface polishing with a mini-brush. Journal Dental Materials. 2009;28(3):277–84.
Butera A, Gallo S, Pascadopoli M, Sfondrini B, Alovisi M, Marya A, Stablum G, Scribante A. Assessment of the bacterial loads of vacuum-thermoformed removable retainers (VTRR) in patients under retention therapy: a randomized clinical trial. Journal of Healthcare. 2022;10(7):1239.
Gunawan V, Carrington SD, Choi YJ, Choi JJ. Air-polishing technology is an effective alternative chairside method for cleaning dentures. International Journal of Dental Hygiene. 2023;22(3)626–638.
Yu R, Zhou Y, Feng H, Liu X. Biocompatibility test of polymethylmethacrylate denture base resin containing silver-supported antimicrobial agent STR-1 at nanometer level. Beijing Da Xue Xue Bao Yi Xue Ban. 2006;38(5):522–24.
Ermetici M. Segù М, Butera А. Comparison to the scanning electron microscope of professional dental hygiene methods on metal-free layered structures and metal-free monolithic structures processed by different polymerization cycles. Minerva Stomatologica. English Italian. 2014;63(6):189–202.
Aykent F, Yondem I, Ozyesil AG, Gunal SK, Avunduk MC, Ozkan S. Effect of different finishing techniques for restorative materials on surface roughness and bacterial adhesion. Journal of Prosthetetic Dentistry. 2010;103(4):221–27.